In vitro control of phytopathogenic fungi and damping-off of tomato by Bacillus velezensis LABIM40 (CMRP 4489)

The in vitro antagonistic activity of Bacillus velezensis LABIM40 (strain CMRP 4489) was assessed against Alternaria linariae, Botryotinia squamosa, Colletotrichum lindemuthianum, Gibberella zeae, and Rhizoctonia solani. An experiment conducted using treated seeds under growth chamber conditions to determine the impact various formulations on tomato seedling biocontrol damping-off caused by R. treatments included use cell suspension, cell-free supernatant (CFS), 10 times concentrated CFS (10× CFS), commercial products based amyloliquefaciens (CP_1) subtilis (CP_2), water. effects these were seedlings grown sterile substrate or inoculated with In a dual culture test, B. inhibited mycelial aforementioned fungal pathogens 46.6%, 67.4%, 64.7%, 49.0%, 54.4%, respectively. minimum inhibitory concentration each fungus determined varying concentrations potato dextrose agar medium, followed regression analysis inhibition. Except for A. logarithmic model provided best fit all cases. Tomato from 10× exhibited survival rate 57% higher than that control treatment. However, no promotion observed plants cells its metabolites. summary, findings highlight C. G. solani, as demonstrated diffusion tests. This suggests potential agent tomatoes.